Because of this, these organisms are frequently targeted for metabolic engineering and synthetic biology. Producing a plethora of medicinally and agriculturally important metabolites, they also serve as biocontrol agents, encode industrial enzymes, and degrade recalcitrant biomass. Streptomyces and related filamentous actinomycetes are widely recognized for their biotechnological value. The online version of this article (10.1007/s10296-7) contains supplementary material, which is available to authorized users. To our knowledge this is the first documentation of conjugation-mediated multiplex plasmid transformation, a useful approach for rapid combinatorial strain development. Importantly, the resulting compatible vector panel enabled us to demonstrate the transfer of up to three plasmids each into Streptomyces venezuelae, Streptomyces roseosporus and Streptomyces pristinaespiralis during a single conjugation experiment. We further report the development of new ϕC31 and ϕBT1-based vectors having orthogonal resistance, replication and integration features for concomitant transformation with our ϕOZJ-derived vectors. using ϕOZJ-derived vectors, we reveal their potential for strain engineering. By demonstrating the transformation of several Streptomyces spp. Towards developing tools for facile multiplex engineering, we herein describe the development of new integrating vectors derived from BD1 subgroup actinophage OzzyJ (ϕOZJ). Among these tools, vectors that enable combinatorial chromosomal manipulations are particularly desirable.
Such efforts rely on the availability of developed molecular toolsets. Streptomyces synthetic biology and genetics empower therapeutic and agrichemical development through strain improvement and biosynthetic understanding. Streptomyces and other closely-related actinobacteria are important sources of bioactive molecules.
0 kommentar(er)
